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fret-based ateam reporter protein  (ATeam Scientific)

 
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    ATeam Scientific fret-based ateam reporter protein
    Comparison of ATP levels determined with <t>FRET-based</t> ATeam reporter proteins in wild-type and mutant yeast strains. Cells were transformed with the different ATeam reporter proteins, grown overnight in oleate medium and transferred to 25 mM MES buffer (pH 6.0) supplemented with 20 g/L glucose prior to measurements. <t>(A)</t> <t>Peroxisomal</t> ATP levels determined with reporter proteins ATeam-per and ATeam-per (mut) (B) ATP levels determined with reporter proteins ATeam-cyt and ATeam-cyt (mut). FRET was determined by measuring 527/475 nm. Ratios were calculated after subtraction of the background signal from wild-type cells transformed with an empty vector. The semi-relative ATP levels were then calculated by subtracting the 572/475 ratio of the ATeam(mut) reporter from the 572/475 of ATeam reporter. Data are means ± SD of values from 4–13 independent experiments. Only most relevant statistic relations are indicated in , all are given in . ****, ***, **, and * indicate significance with a p -value of p < 0.0001, p < 0.001, p < 0.01, and p < 0.05 respectively.
    Fret Based Ateam Reporter Protein, supplied by ATeam Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fret-based+ateam+reporter+protein/pmc08807639-56-22-23?v=ATeam+Scientific
    Average 90 stars, based on 1 article reviews
    fret-based ateam reporter protein - by Bioz Stars, 2026-07
    90/100 stars

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    1) Product Images from "Peroxisomal ATP Uptake Is Provided by Two Adenine Nucleotide Transporters and the ABCD Transporters"

    Article Title: Peroxisomal ATP Uptake Is Provided by Two Adenine Nucleotide Transporters and the ABCD Transporters

    Journal: Frontiers in Cell and Developmental Biology

    doi: 10.3389/fcell.2021.788921

    Comparison of ATP levels determined with FRET-based ATeam reporter proteins in wild-type and mutant yeast strains. Cells were transformed with the different ATeam reporter proteins, grown overnight in oleate medium and transferred to 25 mM MES buffer (pH 6.0) supplemented with 20 g/L glucose prior to measurements. (A) Peroxisomal ATP levels determined with reporter proteins ATeam-per and ATeam-per (mut) (B) ATP levels determined with reporter proteins ATeam-cyt and ATeam-cyt (mut). FRET was determined by measuring 527/475 nm. Ratios were calculated after subtraction of the background signal from wild-type cells transformed with an empty vector. The semi-relative ATP levels were then calculated by subtracting the 572/475 ratio of the ATeam(mut) reporter from the 572/475 of ATeam reporter. Data are means ± SD of values from 4–13 independent experiments. Only most relevant statistic relations are indicated in , all are given in . ****, ***, **, and * indicate significance with a p -value of p < 0.0001, p < 0.001, p < 0.01, and p < 0.05 respectively.
    Figure Legend Snippet: Comparison of ATP levels determined with FRET-based ATeam reporter proteins in wild-type and mutant yeast strains. Cells were transformed with the different ATeam reporter proteins, grown overnight in oleate medium and transferred to 25 mM MES buffer (pH 6.0) supplemented with 20 g/L glucose prior to measurements. (A) Peroxisomal ATP levels determined with reporter proteins ATeam-per and ATeam-per (mut) (B) ATP levels determined with reporter proteins ATeam-cyt and ATeam-cyt (mut). FRET was determined by measuring 527/475 nm. Ratios were calculated after subtraction of the background signal from wild-type cells transformed with an empty vector. The semi-relative ATP levels were then calculated by subtracting the 572/475 ratio of the ATeam(mut) reporter from the 572/475 of ATeam reporter. Data are means ± SD of values from 4–13 independent experiments. Only most relevant statistic relations are indicated in , all are given in . ****, ***, **, and * indicate significance with a p -value of p < 0.0001, p < 0.001, p < 0.01, and p < 0.05 respectively.

    Techniques Used: Comparison, Mutagenesis, Transformation Assay, Plasmid Preparation



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    fret-based ateam reporter protein  (ATeam Scientific)
    90
    ATeam Scientific fret-based ateam reporter protein
    Comparison of ATP levels determined with <t>FRET-based</t> ATeam reporter proteins in wild-type and mutant yeast strains. Cells were transformed with the different ATeam reporter proteins, grown overnight in oleate medium and transferred to 25 mM MES buffer (pH 6.0) supplemented with 20 g/L glucose prior to measurements. <t>(A)</t> <t>Peroxisomal</t> ATP levels determined with reporter proteins ATeam-per and ATeam-per (mut) (B) ATP levels determined with reporter proteins ATeam-cyt and ATeam-cyt (mut). FRET was determined by measuring 527/475 nm. Ratios were calculated after subtraction of the background signal from wild-type cells transformed with an empty vector. The semi-relative ATP levels were then calculated by subtracting the 572/475 ratio of the ATeam(mut) reporter from the 572/475 of ATeam reporter. Data are means ± SD of values from 4–13 independent experiments. Only most relevant statistic relations are indicated in , all are given in . ****, ***, **, and * indicate significance with a p -value of p < 0.0001, p < 0.001, p < 0.01, and p < 0.05 respectively.
    Fret Based Ateam Reporter Protein, supplied by ATeam Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fret-based+ateam+reporter+protein/pmc08807639-56-22-23?v=ATeam+Scientific
    Average 90 stars, based on 1 article reviews
    fret-based ateam reporter protein - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Comparison of ATP levels determined with FRET-based ATeam reporter proteins in wild-type and mutant yeast strains. Cells were transformed with the different ATeam reporter proteins, grown overnight in oleate medium and transferred to 25 mM MES buffer (pH 6.0) supplemented with 20 g/L glucose prior to measurements. (A) Peroxisomal ATP levels determined with reporter proteins ATeam-per and ATeam-per (mut) (B) ATP levels determined with reporter proteins ATeam-cyt and ATeam-cyt (mut). FRET was determined by measuring 527/475 nm. Ratios were calculated after subtraction of the background signal from wild-type cells transformed with an empty vector. The semi-relative ATP levels were then calculated by subtracting the 572/475 ratio of the ATeam(mut) reporter from the 572/475 of ATeam reporter. Data are means ± SD of values from 4–13 independent experiments. Only most relevant statistic relations are indicated in , all are given in . ****, ***, **, and * indicate significance with a p -value of p < 0.0001, p < 0.001, p < 0.01, and p < 0.05 respectively.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Peroxisomal ATP Uptake Is Provided by Two Adenine Nucleotide Transporters and the ABCD Transporters

    doi: 10.3389/fcell.2021.788921

    Figure Lengend Snippet: Comparison of ATP levels determined with FRET-based ATeam reporter proteins in wild-type and mutant yeast strains. Cells were transformed with the different ATeam reporter proteins, grown overnight in oleate medium and transferred to 25 mM MES buffer (pH 6.0) supplemented with 20 g/L glucose prior to measurements. (A) Peroxisomal ATP levels determined with reporter proteins ATeam-per and ATeam-per (mut) (B) ATP levels determined with reporter proteins ATeam-cyt and ATeam-cyt (mut). FRET was determined by measuring 527/475 nm. Ratios were calculated after subtraction of the background signal from wild-type cells transformed with an empty vector. The semi-relative ATP levels were then calculated by subtracting the 572/475 ratio of the ATeam(mut) reporter from the 572/475 of ATeam reporter. Data are means ± SD of values from 4–13 independent experiments. Only most relevant statistic relations are indicated in , all are given in . ****, ***, **, and * indicate significance with a p -value of p < 0.0001, p < 0.001, p < 0.01, and p < 0.05 respectively.

    Article Snippet: To provide in vivo evidence for the role of Ant1p and Pxa1p/Pxa2p in peroxisomal ATP uptake, we expressed modified versions of the FRET-based ATeam reporter protein ( ; ) in the different yeast strains to measure the relative ATP levels in the peroxisomes (ATeam-per), i.e. extended with a carboxy-terminal peroxisomal targeting signal) and the cytosol (ATeam-cyt).

    Techniques: Comparison, Mutagenesis, Transformation Assay, Plasmid Preparation